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primescript rt kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript rt kit
    Primescript Rt Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 11030 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pm42883494-79-5-8
    Average 98 stars, based on 11030 article reviews
    primescript rt kit - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: The copper transporter protein SLC31A1-mediated copper metabolism imbalance drives cuproptosis through the SIRT1/HMGB1 pathway in heart failure.
    Article Snippet: Objective: Disruption of copper (Cu) homeostasis can induce pathological alterations, including heart failure (HF).. Solute carrier family 31 member 1 (SLC31A1) is a significant Cu transporter protein affecting Cu absorption at cell membranes.. This study investigated the role of SLC31A1-regulated Cu metabolism in HF and the underlying

    Article Title: Mettl3-modified lncRNA Snhg16 aggravates osteoarthritis via promoting chondrocyte ferroptosis by regulating Snd1/Gpx4 axis.
    Article Snippet: Purpose/Aim: Long noncoding RNA (lncRNA) small nucleolar RNA host gene 16 (Snhg16) has been confirmed to accelerate osteoarthritis (OA) progress.. However, its regulatory mechanism has not been fully elucidated.. Materials and Methods: Interleukin-1β (IL-1β)-induced ATDC5 chondrocytes were used to mimic OA cell models, and destabilization of medial meniscus (DMM) surgery was performed to construct OA mice models.

    Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis.
    Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined.

    Article Title: Decitabine induces cell differentiation and growth inhibition accompanied by upregulation of microRNA-143 and MS4A3 in the myelodysplastic syndromes-derived leukemic cell line MDS-L.
    Article Snippet: RNA was extracted from cells using a RNeasy Mini Kit (Qiagen Sciences, Inc.). .. Total RNA was reverse transcribed to cDNA using a PrimeScript RT‐PCR kit (Takara Bio, Inc.) and TaqMan MicroRNA Reverse Transcription kit (cat. no. 4366597; Thermo Fisher Scientific, Inc.). .. Quantitative PCR was carried out using a TaqMan Fast Advanced Master Mix kit and Step One Plus real‐time PCR systems (Applied Biosystems; Thermo Fisher Scientific, Inc.) with the following conditions: An initial 95 ̊C for 20 sec, followed by 40 cycles of 95 ̊C for 1 sec and 60 ̊C for 20 sec.

    Article Title: Visualizing intestinal colonization of GFP-displayed Lactobacillus plantarum PO23 and its effects on intestinal morphology and immunity in Paralichthys olivaceus.
    Article Snippet: Visualization of probiotic intestinal colonization is essential for clarifying their adhesion characteristics and mucosal immunomodulatory mechanisms.. GFP surface display enables real-time visualization of probiotic attachment, distribution, and migration dynamics in living hosts.. In our previous study, a highly adhesive potential probiotic strain, Lactobacillus plantarum PO23 (LP-PO23), was isolated from the intestine of flounder (Paralichthys olivaceus).

    Article Title: Apoptotic vesicle-hybridized nanosponges enable dual-phase immunomodulation for targeted periodontitis therapy
    Article Snippet: Total RNA Kit (Omega Bio-Tek, USA, Cat. No. R6834–01) according to the manufacturer's protocol. .. After quantification via UV–Vis spectrophotometry (Nanodrop, Thermo Fisher Scientific, USA), the extracted RNA was reverse-transcribed into cDNA using the PrimeScript RT-PCR Kit (RR037A, TaKaRa, Japan). .. QRT-PCR was performed with the TB Green Premix Ex Taq II kit (Takara, Japan) on the CFX96 qRT-PCR System (Bio-Rad, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The copper transporter protein SLC31A1-mediated copper metabolism imbalance drives cuproptosis through the SIRT1/HMGB1 pathway in heart failure.
    Article Snippet: Objective: Disruption of copper (Cu) homeostasis can induce pathological alterations, including heart failure (HF).. Solute carrier family 31 member 1 (SLC31A1) is a significant Cu transporter protein affecting Cu absorption at cell membranes.. This study investigated the role of SLC31A1-regulated Cu metabolism in HF and the underlying

    Article Title: Mettl3-modified lncRNA Snhg16 aggravates osteoarthritis via promoting chondrocyte ferroptosis by regulating Snd1/Gpx4 axis.
    Article Snippet: Purpose/Aim: Long noncoding RNA (lncRNA) small nucleolar RNA host gene 16 (Snhg16) has been confirmed to accelerate osteoarthritis (OA) progress.. However, its regulatory mechanism has not been fully elucidated.. Materials and Methods: Interleukin-1β (IL-1β)-induced ATDC5 chondrocytes were used to mimic OA cell models, and destabilization of medial meniscus (DMM) surgery was performed to construct OA mice models.

    Article Title: APE1 deficiency drives lens epithelial cell senescence in age-related cataract formation
    Article Snippet: After washing with phosphate-buffered saline, the samples were 208 incubated with Alexa Fluor 488- or 594-conjugated secondary antibodies at room 209 temperature for 2 h. Subsequently, the explants were stained with DAPI for 30 min, 210 flat-mounted on slides, and imaged using a fluorescence microscope (Olympus, 211 IX83). .. 212 213 RNA extraction and quantitative real-time PCR 214 Jo urn al Pr -pr oo f 11 The total RNA from HLE-B3 cells treated with E3330 (200 μM) or APE1-IN-1 (10 215 μM), or from the anterior capsule tissue of the lens obtained from patients with ARC, 216 was extracted using the Simply P Total RNA Extraction Kit (BioFlux) and reverse-217 transcribed with the PrimeScript RT-PCR kit (Takara Biotechnology), following the 218 manufacturer's instructions. ..

    Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis.
    Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined.

    Article Title: Visualizing intestinal colonization of GFP-displayed Lactobacillus plantarum PO23 and its effects on intestinal morphology and immunity in Paralichthys olivaceus.
    Article Snippet: Visualization of probiotic intestinal colonization is essential for clarifying their adhesion characteristics and mucosal immunomodulatory mechanisms.. GFP surface display enables real-time visualization of probiotic attachment, distribution, and migration dynamics in living hosts.. In our previous study, a highly adhesive potential probiotic strain, Lactobacillus plantarum PO23 (LP-PO23), was isolated from the intestine of flounder (Paralichthys olivaceus).

    Article Title: Apoptotic vesicle-hybridized nanosponges enable dual-phase immunomodulation for targeted periodontitis therapy
    Article Snippet: Total RNA Kit (Omega Bio-Tek, USA, Cat. No. R6834–01) according to the manufacturer's protocol. .. After quantification via UV–Vis spectrophotometry (Nanodrop, Thermo Fisher Scientific, USA), the extracted RNA was reverse-transcribed into cDNA using the PrimeScript RT-PCR Kit (RR037A, TaKaRa, Japan). .. QRT-PCR was performed with the TB Green Premix Ex Taq II kit (Takara, Japan) on the CFX96 qRT-PCR System (Bio-Rad, USA).

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    RNA Extraction:

    Article Title: APE1 deficiency drives lens epithelial cell senescence in age-related cataract formation
    Article Snippet: After washing with phosphate-buffered saline, the samples were 208 incubated with Alexa Fluor 488- or 594-conjugated secondary antibodies at room 209 temperature for 2 h. Subsequently, the explants were stained with DAPI for 30 min, 210 flat-mounted on slides, and imaged using a fluorescence microscope (Olympus, 211 IX83). .. 212 213 RNA extraction and quantitative real-time PCR 214 Jo urn al Pr -pr oo f 11 The total RNA from HLE-B3 cells treated with E3330 (200 μM) or APE1-IN-1 (10 215 μM), or from the anterior capsule tissue of the lens obtained from patients with ARC, 216 was extracted using the Simply P Total RNA Extraction Kit (BioFlux) and reverse-217 transcribed with the PrimeScript RT-PCR kit (Takara Biotechnology), following the 218 manufacturer's instructions. ..

    Real-time Polymerase Chain Reaction:

    Article Title: APE1 deficiency drives lens epithelial cell senescence in age-related cataract formation
    Article Snippet: After washing with phosphate-buffered saline, the samples were 208 incubated with Alexa Fluor 488- or 594-conjugated secondary antibodies at room 209 temperature for 2 h. Subsequently, the explants were stained with DAPI for 30 min, 210 flat-mounted on slides, and imaged using a fluorescence microscope (Olympus, 211 IX83). .. 212 213 RNA extraction and quantitative real-time PCR 214 Jo urn al Pr -pr oo f 11 The total RNA from HLE-B3 cells treated with E3330 (200 μM) or APE1-IN-1 (10 215 μM), or from the anterior capsule tissue of the lens obtained from patients with ARC, 216 was extracted using the Simply P Total RNA Extraction Kit (BioFlux) and reverse-217 transcribed with the PrimeScript RT-PCR kit (Takara Biotechnology), following the 218 manufacturer's instructions. ..

    Article Title: Dichloroacetate mitigates experimental abdominal aortic aneurysm via suppression of CXCL5-mediated neutrophil chemotaxis.
    Article Snippet: Background: Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease with high rupture-associated mortality.. Current management relies on surgical or endovascular repair, with no effective pharmacological therapy.. Inflammation and immune cell infiltration are central to AAA pathogenesis, but the molecular mechanisms remain incompletely defined.

    Tissue:

    Article Title: APE1 deficiency drives lens epithelial cell senescence in age-related cataract formation
    Article Snippet: After washing with phosphate-buffered saline, the samples were 208 incubated with Alexa Fluor 488- or 594-conjugated secondary antibodies at room 209 temperature for 2 h. Subsequently, the explants were stained with DAPI for 30 min, 210 flat-mounted on slides, and imaged using a fluorescence microscope (Olympus, 211 IX83). .. 212 213 RNA extraction and quantitative real-time PCR 214 Jo urn al Pr -pr oo f 11 The total RNA from HLE-B3 cells treated with E3330 (200 μM) or APE1-IN-1 (10 215 μM), or from the anterior capsule tissue of the lens obtained from patients with ARC, 216 was extracted using the Simply P Total RNA Extraction Kit (BioFlux) and reverse-217 transcribed with the PrimeScript RT-PCR kit (Takara Biotechnology), following the 218 manufacturer's instructions. ..

    UV-Vis Spectroscopy:

    Article Title: Apoptotic vesicle-hybridized nanosponges enable dual-phase immunomodulation for targeted periodontitis therapy
    Article Snippet: Total RNA Kit (Omega Bio-Tek, USA, Cat. No. R6834–01) according to the manufacturer's protocol. .. After quantification via UV–Vis spectrophotometry (Nanodrop, Thermo Fisher Scientific, USA), the extracted RNA was reverse-transcribed into cDNA using the PrimeScript RT-PCR Kit (RR037A, TaKaRa, Japan). .. QRT-PCR was performed with the TB Green Premix Ex Taq II kit (Takara, Japan) on the CFX96 qRT-PCR System (Bio-Rad, USA).

    Concentration Assay:

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    Spectrophotometry:

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.

    Synthesized:

    Article Title: Dual role of melatonin on Fusarium graminearum in vitro growth and on wheat seedlings infection
    Article Snippet: .. For each sample, according to the manufacturer’s protocols, total RNA was extracted from 100 mg of FG8 mycelium or wheat seedlings using the PureLink RNA Mini Kit (Ambion, Life Technologies, Carlsbad, CA, USA), DNase treatment was performed using the PureLink DNase Set (Ambion, Life Technologies), total RNA concentration was measured using a NanoDrop One (Thermo Fisher Scientific) spectrophotometer and the corresponding single strand cDNA was synthesized from 500 ng of total RNA using PrimeScript RT-PCR Kit (Takara Bio Inc.). .. The Reverse Transcription quantitative PCR (RT-qPCR) was carried out in the CFX Opus 96 Real-Time PCR System (Bio-Rad), using the SYBR Premix Ex Taq II reagent kit (Takara Bio Inc.), according to the manufacturer’s protocols.



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