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one step tb green primescript rt pcr kit ii  (TaKaRa)


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    TaKaRa one step tb green primescript rt pcr kit ii
    One Step Tb Green Primescript Rt Pcr Kit Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+pcr+kit/PrimeScript+RT-PCR+Kit/pmc13107096-64-9-23
    Average 98 stars, based on 10921 article reviews
    one step tb green primescript rt pcr kit ii - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Food Structure Modulation of Antioxidant Bioaccessibility and Gut Epithelial Protection: The Case of Bread and Pasta Made With Pigmented Wheat
    Article Snippet: Total RNA was extracted from the Caco‐2 pellet using the Total RNA Purification Plus Kit (Norgen Biotek, Thorold, ON, Canada), according to the manufacturer's instructions and quantified (NanoDrop, Thermo Fisher Scientific, Italy). .. RNA (1 μg) was retrotranscribed to cDNA using the PrimeScript RT‐PCR Kit (Takara Bio, Göteborg, Sweden), and quantitative real‐time PCR (Biorad CFX96) was used to perform the gene expression analysis using TB Green Premix Ex Taq (Takara Bio, Göteborg, Sweden). ..

    Article Title: GLT8D2-mediated PD-L1 N-glycosylation promotes tumor immune evasion in ovarian cancer peritoneal metastasis.
    Article Snippet: 35 Peritoneal metastasis is the most common form of metastasis in ovarian cancer 36 (OC), resulting in a poor prognosis.. Abnormal modification of proteins by 37 glycosyltransferases is closely related to immunosuppression and tumor 38 metastasis.. However, there were few reports on glycosyltransferase related 39 biomarkers of peritoneal metastasis in OC.

    Article Title: Inhibition of circNUP93 (hsa_circ_0039480) activates the IRS-1/Akt pathway and alleviates insulin resistance in gestational diabetes mellitus via the MiR-767-3p/HIF1A axis.
    Article Snippet: Gestational diabetes mellitus (GDM) is characterized by insulin resistance (IR) and impaired glucose tolerance during pregnancy, yet the upstream regulatory mechanisms controlling insulin signaling remain unclear.. The circular RNA hsa_circ_0039480 (circNUP93) has been proposed as a potential biomarker for GDM, but its functional role in disease progression is poorly understood.. A high-fat diet–induced GDM mouse model and insulin-resistant HTR-8/SVneo trophoblast cell model were used to investigate the role of circNUP93.

    Gene Expression:

    Article Title: Food Structure Modulation of Antioxidant Bioaccessibility and Gut Epithelial Protection: The Case of Bread and Pasta Made With Pigmented Wheat
    Article Snippet: Total RNA was extracted from the Caco‐2 pellet using the Total RNA Purification Plus Kit (Norgen Biotek, Thorold, ON, Canada), according to the manufacturer's instructions and quantified (NanoDrop, Thermo Fisher Scientific, Italy). .. RNA (1 μg) was retrotranscribed to cDNA using the PrimeScript RT‐PCR Kit (Takara Bio, Göteborg, Sweden), and quantitative real‐time PCR (Biorad CFX96) was used to perform the gene expression analysis using TB Green Premix Ex Taq (Takara Bio, Göteborg, Sweden). ..

    Article Title: Inhibition of circNUP93 (hsa_circ_0039480) activates the IRS-1/Akt pathway and alleviates insulin resistance in gestational diabetes mellitus via the MiR-767-3p/HIF1A axis.
    Article Snippet: Gestational diabetes mellitus (GDM) is characterized by insulin resistance (IR) and impaired glucose tolerance during pregnancy, yet the upstream regulatory mechanisms controlling insulin signaling remain unclear.. The circular RNA hsa_circ_0039480 (circNUP93) has been proposed as a potential biomarker for GDM, but its functional role in disease progression is poorly understood.. A high-fat diet–induced GDM mouse model and insulin-resistant HTR-8/SVneo trophoblast cell model were used to investigate the role of circNUP93.

    Reverse Transcription:

    Article Title: GLT8D2-mediated PD-L1 N-glycosylation promotes tumor immune evasion in ovarian cancer peritoneal metastasis.
    Article Snippet: 35 Peritoneal metastasis is the most common form of metastasis in ovarian cancer 36 (OC), resulting in a poor prognosis.. Abnormal modification of proteins by 37 glycosyltransferases is closely related to immunosuppression and tumor 38 metastasis.. However, there were few reports on glycosyltransferase related 39 biomarkers of peritoneal metastasis in OC.

    Article Title: Extracellular matrix stiffness orchestrates NETs formation and mTOR-Driven metabolic reprogramming in lung cancer.
    Article Snippet: Lung cancer is strongly associated with increased extracellular matrix (ECM) stiffness, which correlates with poor patient prognosis.. Our study reveals that high-stiffness tumor niches exhibit significant upregulation of neutrophil extracellular traps (NETs), which enhance the Warburg effect and promote tumor cell proliferation.. Using atomic force microscopy (AFM) and multi-immunofluorescence staining (mIF), we demonstrated a spatial correlation between NETs formation and localized ECM stiffness in lung cancer tissues.

    Article Title: The osteogenic potential of autogenous cortical bone debris in repairing rabbit calvarial defect: a preclinical study
    Article Snippet: Total RNA was isolated using TRIzol Reagent (Invitrogen, U.S.A.). .. The concentration and purity of RNA were determined spectrophotometrically before reverse transcription, and only qualified RNA samples were used for subsequent analysis. cDNA was synthesized using PrimeScript RT-PCR kit (Takara, Japan). ..

    Article Title: Inhibition of circNUP93 (hsa_circ_0039480) activates the IRS-1/Akt pathway and alleviates insulin resistance in gestational diabetes mellitus via the MiR-767-3p/HIF1A axis.
    Article Snippet: Gestational diabetes mellitus (GDM) is characterized by insulin resistance (IR) and impaired glucose tolerance during pregnancy, yet the upstream regulatory mechanisms controlling insulin signaling remain unclear.. The circular RNA hsa_circ_0039480 (circNUP93) has been proposed as a potential biomarker for GDM, but its functional role in disease progression is poorly understood.. A high-fat diet–induced GDM mouse model and insulin-resistant HTR-8/SVneo trophoblast cell model were used to investigate the role of circNUP93.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: GLT8D2-mediated PD-L1 N-glycosylation promotes tumor immune evasion in ovarian cancer peritoneal metastasis.
    Article Snippet: 35 Peritoneal metastasis is the most common form of metastasis in ovarian cancer 36 (OC), resulting in a poor prognosis.. Abnormal modification of proteins by 37 glycosyltransferases is closely related to immunosuppression and tumor 38 metastasis.. However, there were few reports on glycosyltransferase related 39 biomarkers of peritoneal metastasis in OC.

    Article Title: Extracellular matrix stiffness orchestrates NETs formation and mTOR-Driven metabolic reprogramming in lung cancer.
    Article Snippet: Lung cancer is strongly associated with increased extracellular matrix (ECM) stiffness, which correlates with poor patient prognosis.. Our study reveals that high-stiffness tumor niches exhibit significant upregulation of neutrophil extracellular traps (NETs), which enhance the Warburg effect and promote tumor cell proliferation.. Using atomic force microscopy (AFM) and multi-immunofluorescence staining (mIF), we demonstrated a spatial correlation between NETs formation and localized ECM stiffness in lung cancer tissues.

    Article Title: The osteogenic potential of autogenous cortical bone debris in repairing rabbit calvarial defect: a preclinical study
    Article Snippet: Total RNA was isolated using TRIzol Reagent (Invitrogen, U.S.A.). .. The concentration and purity of RNA were determined spectrophotometrically before reverse transcription, and only qualified RNA samples were used for subsequent analysis. cDNA was synthesized using PrimeScript RT-PCR kit (Takara, Japan). ..

    Article Title: Inhibition of circNUP93 (hsa_circ_0039480) activates the IRS-1/Akt pathway and alleviates insulin resistance in gestational diabetes mellitus via the MiR-767-3p/HIF1A axis.
    Article Snippet: Gestational diabetes mellitus (GDM) is characterized by insulin resistance (IR) and impaired glucose tolerance during pregnancy, yet the upstream regulatory mechanisms controlling insulin signaling remain unclear.. The circular RNA hsa_circ_0039480 (circNUP93) has been proposed as a potential biomarker for GDM, but its functional role in disease progression is poorly understood.. A high-fat diet–induced GDM mouse model and insulin-resistant HTR-8/SVneo trophoblast cell model were used to investigate the role of circNUP93.

    Article Title: TBX20 regulates epithelial-mesenchymal transition, maintains tumor stemness and immune factor expression, and promotes immune escape in lung cancer
    Article Snippet: The following antibodies were used in the present study: TBX20 (cat. no. 83414-5-RR; 1:1,000; Wuhan Sanying Biotechnology), E-cadherin (cat. no. 60335-1-Ig; 1:1,000; Wuhan Sanying Biotechnology), N-cadherin (cat. no. 66219-1-Ig; 1:2,000; Wuhan Sanying Biotechnology), vimentin (cat. no. A19607; 1:1,000; ABclonal Biotech Co., Ltd.), CD44 (cat. no. 84854-5-RR; 1:500; Wuhan Sanying Biotechnology), Sox2 (cat. no. 66411-1-Ig; 1:500; Wuhan Sanying Biotechnology), Nanog (cat. no. 14295-1-AP; 1:500; Wuhan Sanying Biotechnology), PD-L1 (cat. no. 66248-1-Ig; 1:100; Wuhan Sanying Biotechnology), IL-6 (cat. no. A0286; 1:200; ABclonal Biotech Co., Ltd.), CCL2 (cat. no. MA5-17040; 1:500; Thermo Fisher Scientific, Inc.) and β-actin (cat. no. 81115-1-RR; 1:2,000; Wuhan Sanying Biotechnology) primary antibodies, and HRP-conjugated rabbit anti-mouse and goat anti-rabbit secondary antibodies (cat. nos. ab6728 and ab6721; 1:5,000; Abcam). .. Total RNA was extracted from A549 and HCI-H1975 cells using a TRIzol kit (Invitrogen), and the RNA concentration and purity were measured with a NanoDrop microplate spectrophotometer (Thermo Fisher Scientific, Inc.). cDNA was subsequently synthesized by RT using a PrimeScript RT-PCR kit (Takara Bio, Inc.), performed according to manufacturer's protocol. .. Finally, qPCR was performed on cDNA using the eQ9600 real-time fluorescent qPCR detection system (Suzhou Dongsheng Xingye Scientific Instruments Co., Ltd.), Thermocycling conditions were as follows: Pre-denaturation at 95°C for 3 min; Denaturation at 95°C for 5 sec, annealing at 60°C for 30 sec, extension at 72°C for 20 sec, a total of 40 cycles.

    Article Title: CD36/ANXA1/TLR4/NF-κB Axis Orchestrates Lipid Metabolism, Chronic Inflammation, and Insulin Resistance in Obese Children.
    Article Snippet: Background: Childhood obesity is tightly linked to dyslipidemia, chronic low-grade inflammation, and insulin resistance (IR).. CD36 has been implicated in metabolic disease, yet its pediatric mechanisms remain unclear.. This study aims to investigate the CD36/ANXA1/TLR4/NF-κB axis as a potential therapeutic target for lipid metabolism and IR in childhood obesity.

    Article Title: HIF-mediated regulation of glutathione-specific gamma-glutamyl cyclotransferase 1 contributes to tubular cell death in renal ischemia-reperfusion.
    Article Snippet: .. One mg of total RNA was subjected to RT-PCR using a PrimeScript RT-PCR Kit with Oligo dT Primer (Takara). ..

    Concentration Assay:

    Article Title: The osteogenic potential of autogenous cortical bone debris in repairing rabbit calvarial defect: a preclinical study
    Article Snippet: Total RNA was isolated using TRIzol Reagent (Invitrogen, U.S.A.). .. The concentration and purity of RNA were determined spectrophotometrically before reverse transcription, and only qualified RNA samples were used for subsequent analysis. cDNA was synthesized using PrimeScript RT-PCR kit (Takara, Japan). ..

    Article Title: TBX20 regulates epithelial-mesenchymal transition, maintains tumor stemness and immune factor expression, and promotes immune escape in lung cancer
    Article Snippet: The following antibodies were used in the present study: TBX20 (cat. no. 83414-5-RR; 1:1,000; Wuhan Sanying Biotechnology), E-cadherin (cat. no. 60335-1-Ig; 1:1,000; Wuhan Sanying Biotechnology), N-cadherin (cat. no. 66219-1-Ig; 1:2,000; Wuhan Sanying Biotechnology), vimentin (cat. no. A19607; 1:1,000; ABclonal Biotech Co., Ltd.), CD44 (cat. no. 84854-5-RR; 1:500; Wuhan Sanying Biotechnology), Sox2 (cat. no. 66411-1-Ig; 1:500; Wuhan Sanying Biotechnology), Nanog (cat. no. 14295-1-AP; 1:500; Wuhan Sanying Biotechnology), PD-L1 (cat. no. 66248-1-Ig; 1:100; Wuhan Sanying Biotechnology), IL-6 (cat. no. A0286; 1:200; ABclonal Biotech Co., Ltd.), CCL2 (cat. no. MA5-17040; 1:500; Thermo Fisher Scientific, Inc.) and β-actin (cat. no. 81115-1-RR; 1:2,000; Wuhan Sanying Biotechnology) primary antibodies, and HRP-conjugated rabbit anti-mouse and goat anti-rabbit secondary antibodies (cat. nos. ab6728 and ab6721; 1:5,000; Abcam). .. Total RNA was extracted from A549 and HCI-H1975 cells using a TRIzol kit (Invitrogen), and the RNA concentration and purity were measured with a NanoDrop microplate spectrophotometer (Thermo Fisher Scientific, Inc.). cDNA was subsequently synthesized by RT using a PrimeScript RT-PCR kit (Takara Bio, Inc.), performed according to manufacturer's protocol. .. Finally, qPCR was performed on cDNA using the eQ9600 real-time fluorescent qPCR detection system (Suzhou Dongsheng Xingye Scientific Instruments Co., Ltd.), Thermocycling conditions were as follows: Pre-denaturation at 95°C for 3 min; Denaturation at 95°C for 5 sec, annealing at 60°C for 30 sec, extension at 72°C for 20 sec, a total of 40 cycles.

    Synthesized:

    Article Title: The osteogenic potential of autogenous cortical bone debris in repairing rabbit calvarial defect: a preclinical study
    Article Snippet: Total RNA was isolated using TRIzol Reagent (Invitrogen, U.S.A.). .. The concentration and purity of RNA were determined spectrophotometrically before reverse transcription, and only qualified RNA samples were used for subsequent analysis. cDNA was synthesized using PrimeScript RT-PCR kit (Takara, Japan). ..

    Article Title: TBX20 regulates epithelial-mesenchymal transition, maintains tumor stemness and immune factor expression, and promotes immune escape in lung cancer
    Article Snippet: The following antibodies were used in the present study: TBX20 (cat. no. 83414-5-RR; 1:1,000; Wuhan Sanying Biotechnology), E-cadherin (cat. no. 60335-1-Ig; 1:1,000; Wuhan Sanying Biotechnology), N-cadherin (cat. no. 66219-1-Ig; 1:2,000; Wuhan Sanying Biotechnology), vimentin (cat. no. A19607; 1:1,000; ABclonal Biotech Co., Ltd.), CD44 (cat. no. 84854-5-RR; 1:500; Wuhan Sanying Biotechnology), Sox2 (cat. no. 66411-1-Ig; 1:500; Wuhan Sanying Biotechnology), Nanog (cat. no. 14295-1-AP; 1:500; Wuhan Sanying Biotechnology), PD-L1 (cat. no. 66248-1-Ig; 1:100; Wuhan Sanying Biotechnology), IL-6 (cat. no. A0286; 1:200; ABclonal Biotech Co., Ltd.), CCL2 (cat. no. MA5-17040; 1:500; Thermo Fisher Scientific, Inc.) and β-actin (cat. no. 81115-1-RR; 1:2,000; Wuhan Sanying Biotechnology) primary antibodies, and HRP-conjugated rabbit anti-mouse and goat anti-rabbit secondary antibodies (cat. nos. ab6728 and ab6721; 1:5,000; Abcam). .. Total RNA was extracted from A549 and HCI-H1975 cells using a TRIzol kit (Invitrogen), and the RNA concentration and purity were measured with a NanoDrop microplate spectrophotometer (Thermo Fisher Scientific, Inc.). cDNA was subsequently synthesized by RT using a PrimeScript RT-PCR kit (Takara Bio, Inc.), performed according to manufacturer's protocol. .. Finally, qPCR was performed on cDNA using the eQ9600 real-time fluorescent qPCR detection system (Suzhou Dongsheng Xingye Scientific Instruments Co., Ltd.), Thermocycling conditions were as follows: Pre-denaturation at 95°C for 3 min; Denaturation at 95°C for 5 sec, annealing at 60°C for 30 sec, extension at 72°C for 20 sec, a total of 40 cycles.

    Article Title: CD36/ANXA1/TLR4/NF-κB Axis Orchestrates Lipid Metabolism, Chronic Inflammation, and Insulin Resistance in Obese Children.
    Article Snippet: Background: Childhood obesity is tightly linked to dyslipidemia, chronic low-grade inflammation, and insulin resistance (IR).. CD36 has been implicated in metabolic disease, yet its pediatric mechanisms remain unclear.. This study aims to investigate the CD36/ANXA1/TLR4/NF-κB axis as a potential therapeutic target for lipid metabolism and IR in childhood obesity.

    Extraction:

    Article Title: Inhibition of circNUP93 (hsa_circ_0039480) activates the IRS-1/Akt pathway and alleviates insulin resistance in gestational diabetes mellitus via the MiR-767-3p/HIF1A axis.
    Article Snippet: Gestational diabetes mellitus (GDM) is characterized by insulin resistance (IR) and impaired glucose tolerance during pregnancy, yet the upstream regulatory mechanisms controlling insulin signaling remain unclear.. The circular RNA hsa_circ_0039480 (circNUP93) has been proposed as a potential biomarker for GDM, but its functional role in disease progression is poorly understood.. A high-fat diet–induced GDM mouse model and insulin-resistant HTR-8/SVneo trophoblast cell model were used to investigate the role of circNUP93.

    Isolation:

    Article Title: Inhibition of circNUP93 (hsa_circ_0039480) activates the IRS-1/Akt pathway and alleviates insulin resistance in gestational diabetes mellitus via the MiR-767-3p/HIF1A axis.
    Article Snippet: Gestational diabetes mellitus (GDM) is characterized by insulin resistance (IR) and impaired glucose tolerance during pregnancy, yet the upstream regulatory mechanisms controlling insulin signaling remain unclear.. The circular RNA hsa_circ_0039480 (circNUP93) has been proposed as a potential biomarker for GDM, but its functional role in disease progression is poorly understood.. A high-fat diet–induced GDM mouse model and insulin-resistant HTR-8/SVneo trophoblast cell model were used to investigate the role of circNUP93.

    cDNA Synthesis:

    Article Title: Inhibition of circNUP93 (hsa_circ_0039480) activates the IRS-1/Akt pathway and alleviates insulin resistance in gestational diabetes mellitus via the MiR-767-3p/HIF1A axis.
    Article Snippet: Gestational diabetes mellitus (GDM) is characterized by insulin resistance (IR) and impaired glucose tolerance during pregnancy, yet the upstream regulatory mechanisms controlling insulin signaling remain unclear.. The circular RNA hsa_circ_0039480 (circNUP93) has been proposed as a potential biomarker for GDM, but its functional role in disease progression is poorly understood.. A high-fat diet–induced GDM mouse model and insulin-resistant HTR-8/SVneo trophoblast cell model were used to investigate the role of circNUP93.

    Spectrophotometry:

    Article Title: TBX20 regulates epithelial-mesenchymal transition, maintains tumor stemness and immune factor expression, and promotes immune escape in lung cancer
    Article Snippet: The following antibodies were used in the present study: TBX20 (cat. no. 83414-5-RR; 1:1,000; Wuhan Sanying Biotechnology), E-cadherin (cat. no. 60335-1-Ig; 1:1,000; Wuhan Sanying Biotechnology), N-cadherin (cat. no. 66219-1-Ig; 1:2,000; Wuhan Sanying Biotechnology), vimentin (cat. no. A19607; 1:1,000; ABclonal Biotech Co., Ltd.), CD44 (cat. no. 84854-5-RR; 1:500; Wuhan Sanying Biotechnology), Sox2 (cat. no. 66411-1-Ig; 1:500; Wuhan Sanying Biotechnology), Nanog (cat. no. 14295-1-AP; 1:500; Wuhan Sanying Biotechnology), PD-L1 (cat. no. 66248-1-Ig; 1:100; Wuhan Sanying Biotechnology), IL-6 (cat. no. A0286; 1:200; ABclonal Biotech Co., Ltd.), CCL2 (cat. no. MA5-17040; 1:500; Thermo Fisher Scientific, Inc.) and β-actin (cat. no. 81115-1-RR; 1:2,000; Wuhan Sanying Biotechnology) primary antibodies, and HRP-conjugated rabbit anti-mouse and goat anti-rabbit secondary antibodies (cat. nos. ab6728 and ab6721; 1:5,000; Abcam). .. Total RNA was extracted from A549 and HCI-H1975 cells using a TRIzol kit (Invitrogen), and the RNA concentration and purity were measured with a NanoDrop microplate spectrophotometer (Thermo Fisher Scientific, Inc.). cDNA was subsequently synthesized by RT using a PrimeScript RT-PCR kit (Takara Bio, Inc.), performed according to manufacturer's protocol. .. Finally, qPCR was performed on cDNA using the eQ9600 real-time fluorescent qPCR detection system (Suzhou Dongsheng Xingye Scientific Instruments Co., Ltd.), Thermocycling conditions were as follows: Pre-denaturation at 95°C for 3 min; Denaturation at 95°C for 5 sec, annealing at 60°C for 30 sec, extension at 72°C for 20 sec, a total of 40 cycles.



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    TaKaRa one step sybr primescripttm rt pcr kit
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    One Step Sybr Primescripttm Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+pcr+kit/One+Step+PrimeScript+RT-PCR+Kit/pm42134762-109-6-14
    Average 97 stars, based on 1 article reviews
    one step sybr primescripttm rt pcr kit - by Bioz Stars, 2026-09
    97/100 stars
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    primescript rt pcr master mix kit  (TaKaRa)
    99
    TaKaRa primescript rt pcr master mix kit
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
    Primescript Rt Pcr Master Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+pcr+kit/PrimeScript+RT+Master+Mix/pm42129801-331-18-23
    Average 99 stars, based on 1 article reviews
    primescript rt pcr master mix kit - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Journal: Veterinary Research

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves

    doi: 10.1186/s13567-026-01765-3

    Figure Lengend Snippet: Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Article Snippet: The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction.

    Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification, Marker, Generated, Clone Assay, Construct, Recombinant, Negative Control, Control